通过生测实验发现,在敏感性种群中,携带CCYV的烟粉虱对吡虫啉的敏感度下降,即在同样吡虫啉浓度下,携带CCYV的烟粉虱死亡率显著下降(Fig2)。进一步通过qRT-PCR研究发现,烟粉虱携带CCYV 10 d后,其抗吡虫啉相关基因CYP6CM1及其上游调控因子(p38/CREB)有上调趋势(Fig3,Fig4)。
进一步用RNAi技术沉默了烟粉虱抗性基因CYP6CM1,发现沉默该基因后,与对照相比,烟粉虱的获毒量在1 h,3 h显著下降(Fig5)。我们也比较了抗性和敏感性烟粉虱获毒、持毒能力,发现抗性种群烟粉虱可以快速获取病毒,同时持毒能力相对较短(Fig6),说明抗性烟粉虱可以快速获毒并将病毒传播到新的寄主上。
论文全文链接:https://doi.org/10.1007/s10340-022-01553-w
论文PDF: https://link.springer.com/content/pdf/10.1007/s10340-022-01553-w.pdf.
近年来,闫凤鸣教授团队在烟粉虱传毒及病毒-介体-植物互作机理方面取得系列进展,发表了多篇研究论文,近两年(2021-2022)的论文主要有:
Zhang ZL, Zhang BB, Yan MH, He HF, Li JJ, Yan FM*, How does vector Bemisia tabaci use visual and olfactory cues in orientation to a virus-infected host plant? Frontiers in Ecology and Evolution, 2022 10:766570. doi: 10.3389/fevo.2022.766570
Zhang ZL, He HF, Yan MH, Zhao CC, Lei CY, Li JJ, Yan FM*. Widely targeted analysis of metabolomic changes of Cucumis sativus induced by Cucurbit chlorotic yellows virus. BMC Plant Biology. 2022. 22:158 https://doi.org/10.1186/s12870-022-03555-3.
He HF, Li JJ, Zhang ZL, Tang XF, Song DY and Yan FM*. Impacts of Cucurbit chlorotic yellows virus (CCYV) on bBiological characteristics of Its vector Bemisia tabaci (Hemiptera: Aleyrodidae) MED Species. Journal of Insect Science, 2021, 21(5): 12; 1-7. https://doi.org/10.1093/jisesa/ieab084.
Lu SH, Li JJ, Bai RE, Yan FM*. EPG-recorded feeding behaviors reveal adaptability and competitiveness in two species of Bemisia tabaci (Hemiptera: Aleyrodidae). Journal of Insect Behavior, 2021. 34(1): 26-40. DOI: 10.1007/s10905-021-09765-1